コレクション oil red o staining protocol for cytology 121227-Oil red o staining protocol for cytology
Oil Red O Staining And Morphological Changes In Dfat Gfp And 3t3 L1 Download Scientific Diagram Add Oil Red O working solution for 10 min (do not touch walls of the wells) Remove all Oil Red O and IMMEDIATELY adddH2O, wash with H2O 4 times (you can wash under running tap water) Take picktures if desired Remove all water and let dry Elute Oil Red O by adding 100% isopropanol, incubate about 10 m in (can be longer)Protocol Wash Cells 2X with 1xPBS Remove media by carefully aspirating Do not touch cells (leave the chambers on!) Oil Red O Lipid Staining 10 min Oil Red O is kept as 04% Oil Red in 100% Isopropanol; Oil red o staining protocol for cytology